LC-MS/MS is a direct analytical method based on the molecular weight, structure and other chemical properties of the biomarker to be measured, with multiple reaction monitoring (MRM) as the central technology.LC-MS/MS combines the high separation capability of liquid chromatography with the exclusive specificity of quadrupole tandem mass spectrometry, allowing real-time and quantitative analysis of complex samples. quantitative analysis of complex samples. Even in the case of poor LC separation, as long as the target compounds are scanned by MS1 and MS2 for neutral fragments and matched to specific fragment ions, the target compounds in the sample can be found and highlighted, significantly increasing the sensitivity signal (S/N) and enabling rapid and accurate quantification of the target compounds in complex sample systems.

LC-MS/MS technology enables the simultaneous determination of multiple analytes, which is of great significance for health assessment and disease diagnosis, and represents one of the directions for the development of precision medicine today. Based on many advantages such as high specificity, high sensitivity, low detection limit, small sample size, high throughput and fast detection speed, LC-MS/MS has been commonly used in clinical laboratory tests, such as newborn genetic metabolic disease screening, therapeutic drug monitoring, water-soluble/fat-soluble vitamin analysis, endocrine hormone detection, amino acid, bile acid and peptide protein biomarkers, etc., and has shown a great vitality.